amiE [2017-8-11 16:0:3]

N-acetylmuramyl-L-alanine amidase

locus: BSU01670

pI: 9.35

mw: 49.14 kDa

protein length: 441 aa

gene length: 1323 bp

function: recycling of cell wall material

product: N-acetylmuramyl-L-alanine amidase

essential: no

synonyms: ybbE

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.3|Utilization of cell wall components]

Gene

Coordinates: 188,408 → 189,733

The protein

Catalyzed reaction/ biological activity

• hydrolyzes the amide bond between MurNAc and the L-alanine residue of the stem peptide [Pubmed|20400549]

Protein family

• UPF0214 family (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|0B1F43F43A4DC1574A153DBFBAA5D0A1855F9B61|murQ]-[gene|125872506C400CB6B3DAEA8B5130B064DFBA4494|murR]-[gene|178D5E2AA1225FE909E6F2B63B3595F5ABB8E7A2|murP]-[gene|DEACFD435DB01C4B3ADEF397BEE08E2E7C7FCC36|amiE]-[gene|A5D4BF262BD365DCEBA9358C8DB295E605DE4D84|nagZ]-[gene|005D37F34CA3D6891F7D8FF7A2AFA1CBCB556FFE|ybbC]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [protein|125872506C400CB6B3DAEA8B5130B064DFBA4494|MurR]: repression, [pubmed|30038046], in [regulon|125872506C400CB6B3DAEA8B5130B064DFBA4494|MurR regulon]

  regulation

  • expressed in late exponential and early stationary phase [Pubmed|20400549]

References

• 10627040,20400549,22383849